- Two day advanced course in ‘Liquid Chromatography’
- Two Day course on ‘Introduction to LC/MS and Quantification using LC-MS/MS’
- Three day course on ‘GLP Bioanalysis using LC-MS/MS’
Plus customized training modules on Chromatography and Mass Spectrometry
- Introduction to chromatography and chromatographic process-four modes of chromatography - reversed-phase, normal phase, ion exchange and size exclusion - main focus on reversed-phase LC - the concepts of efficiency, retention factor and selectivity.
- Instrument operation- each part of an HPLC instrument in detail – including the solvent delivery system, sample injection, connecting tubing and fittings, commonly used detectors.
- Chromatograms - significant aspects of the chromatogram such as column void volume and peak efficiency (theoretical plates) and brief description of theory.
- Columns - The heart of chromatography and complete details - modern reversed-phase HPLC columns and different manufacturers - maintenance of columns and cleaning procedures.
- Mobile phase - retention and separation -commonly used solvents and their strength - to adjust solvent conditions to get satisfactory retention - Isocratic and gradient elution.
- Method development - The principles of method development and a systematic approach to developing effective, robust HPLC methods in the most efficient manner, temperature and pH effects and so on.
- Few examples to demonstrate efficient method development.
- Instrument maintenance - Important steps to proper maintenance of an instrument.
- Basics of MS - Requirements of interface to couple LC with MS, vacuum system, different types on interfaces focusing mainly on ESI, API, APPI and other recent interfaces.
- Ionisation methods, analysers and detectors of MS.
- Recording of MS spectrum and over tuning methodology of tuning mass spectrometer, calibration and sensitivity detection for over all LC/MS as a total system. What is MS/MS? How do we get MS/MS of a compound?
- Method development tools using LC/MS.
- Tools to understand a mass spectrum and data interpretation.
- Structural elucidation of an unknown compound using a mass spectrum with examples.
- Characterisation of metabolites using mass spectral data and proposing metabolite pathways.
- LC/MS method validation and quantification.
- Case studies and examples (a) structural elucidation and (b) quantification by LC/MS/MS.
- Analytical troubleshooting in LC/MS and proactive maintenance of LC/MS.
- Recent developments in mass spectrometry.